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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
03/03/2015 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DIAS, N. L.; FONSECA JÚNIOR, A. A.; OLIVEIRA, A. M.; SALES, É. B.; ALVES, B. R. C.; DORELLA, F. A.; CAMARGOS, M. F. |
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Afiliação: |
NATANAEL LAMAS DIAS, LANAGRO - MG; ANTÔNIO AUGUSTO FONSECA JÚNIOR, LANAGRO - MG; ANAPOLINO MACEDO OLIVEIRA, LANAGRO - MG; ÉRICA BRAVO SALES, LANAGRO - MG; BRUNA RIOS COELHO ALVES, CNPGL; FERNANDA ALVES DORELLA, LANAGRO - MG; MARCELO FERNANDES CAMARGOS, LANAGRO - MG. |
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Título: |
Validation of a real time PCR for classical swine fever diagnosis. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Veterinary Medicine International, 2014. |
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Páginas: |
4 p. |
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Idioma: |
Inglês |
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Conteúdo: |
The viral disease classical swine fever (CSF), caused by a Pestivirus, is one of the major causes of economic losses for pig farming.The aim of this work was to validate a RT-qPCR using Taqman for detection of CSF in swine tissues. The parameters for the validation followed the specifications of the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals of the World Organization for Animal Health (OIE) and the guide ABNT NBR ISO/IEC 17025:2005. The analysis of the 5' NTR region of CSF virus was performed in 145 samples from 29 infected pigs and in 240 samples from 80 pigs originated in the Brazilian CSF-free zone. The tissues tested were spleen, kidney, blood, tonsils, and lymph nodes. Sequencing of the positive samples for 5'NTR region was performed to evaluate the specificity of the RT-qPCR. Tests performed for the RT-qPCR validation demonstrated that the PCR assay was efficient in detecting RNA from CSF virus in all materials from different tissues of infected animals. Furthermore, RNA from CSF virus was not detected in samples of swine originated from the Brazilian CSF-free zone. Hence, it is concluded that RT-qPCR can be used as a complementary diagnostic for CSF. |
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Palavras-Chave: |
Classical Swine Fever; PCR; Swine health. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/119249/1/Cnpgl-2014-VetMedInt-Validation.pdf
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Marc: |
LEADER 01851naa a2200241 a 4500
001 2010526
005 2024-02-05
008 2014 bl uuuu u00u1 u #d
100 1 $aDIAS, N. L.
245 $aValidation of a real time PCR for classical swine fever diagnosis.$h[electronic resource]
260 $c2014
300 $a4 p.
520 $aThe viral disease classical swine fever (CSF), caused by a Pestivirus, is one of the major causes of economic losses for pig farming.The aim of this work was to validate a RT-qPCR using Taqman for detection of CSF in swine tissues. The parameters for the validation followed the specifications of the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals of the World Organization for Animal Health (OIE) and the guide ABNT NBR ISO/IEC 17025:2005. The analysis of the 5' NTR region of CSF virus was performed in 145 samples from 29 infected pigs and in 240 samples from 80 pigs originated in the Brazilian CSF-free zone. The tissues tested were spleen, kidney, blood, tonsils, and lymph nodes. Sequencing of the positive samples for 5'NTR region was performed to evaluate the specificity of the RT-qPCR. Tests performed for the RT-qPCR validation demonstrated that the PCR assay was efficient in detecting RNA from CSF virus in all materials from different tissues of infected animals. Furthermore, RNA from CSF virus was not detected in samples of swine originated from the Brazilian CSF-free zone. Hence, it is concluded that RT-qPCR can be used as a complementary diagnostic for CSF.
653 $aClassical Swine Fever
653 $aPCR
653 $aSwine health
700 1 $aFONSECA JÚNIOR, A. A.
700 1 $aOLIVEIRA, A. M.
700 1 $aSALES, É. B.
700 1 $aALVES, B. R. C.
700 1 $aDORELLA, F. A.
700 1 $aCAMARGOS, M. F.
773 $tVeterinary Medicine International, 2014.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
02/08/2012 |
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Data da última atualização: |
10/10/2012 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
BIANCO, L. F.; CARVALHO, J. F. C.; TERASSI, F. S.; SEINO, Y. W.; TREVIZAN, F. H.; ONOFRE, E.; NEUMAIER, N.; OLIVEIRA, M. C. N. de; MARCELINO-GUIMARÃES, F. C.; FARIAS, J. R. B.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L. |
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Afiliação: |
UNOPAR - CNPq/PIBIC; CAPES; UNOPAR - CNPQ/PIBIC; UNOPAR - CNPq/PIBIC; UNOPAR - CNPq/PIBIC; UEL; NORMAN NEUMAIER, CNPSO; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; FRANCISMAR CORREA MARCELINO, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; JIRCAS; ALEXANDRE LIMA NEPOMUCENO, SRI. |
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Título: |
Crescimento de soja geneticamente modificada com os genes AtDREB1A e AtDREB2A sob déficit hídrico. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 7., 2012, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2012. p. 108-114. (Embrapa Soja. Documentos, 333). Editado por Paula Gerón Saiz de Mello. Disponível em: . |
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Idioma: |
Português |
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Palavras-Chave: |
Soja - déficit hídrico. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/67790/1/ID-33365.pdf
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Marc: |
LEADER 00996nam a2200241 a 4500
001 1930303
005 2012-10-10
008 2012 bl uuuu u00u1 u #d
100 1 $aBIANCO, L. F.
245 $aCrescimento de soja geneticamente modificada com os genes AtDREB1A e AtDREB2A sob déficit hídrico.$h[electronic resource]
260 $aIn: JORNADA ACADÊMICA DA EMBRAPA SOJA, 7., 2012, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2012. p. 108-114. (Embrapa Soja. Documentos, 333). Editado por Paula Gerón Saiz de Mello. Disponível em: <http://www.cnpso.embrapa.br/download/Doc-333.pdf>.$c2012
653 $aSoja - déficit hídrico
700 1 $aCARVALHO, J. F. C.
700 1 $aTERASSI, F. S.
700 1 $aSEINO, Y. W.
700 1 $aTREVIZAN, F. H.
700 1 $aONOFRE, E.
700 1 $aNEUMAIER, N.
700 1 $aOLIVEIRA, M. C. N. de
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aFARIAS, J. R. B.
700 1 $aYAMAGUCHI-SHINOZAKI, K.
700 1 $aNEPOMUCENO, A. L.
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Embrapa Soja (CNPSO) |
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